Dear all,
We are attempting to clone into pETDuet, pACYCDuet, pCola1Duet and pCDFDuet
and we are encountering numerous difficulties.
We are aware that most of them are low copy number, but even taking this
into account, we find it puzzling that whereas the first preps of the
vectors gave low, but significant amount of DNA, as time goes by the
quantities we get are constantly decreasing. And any subsequent step
(ligation, etc.) is a real challenge.
Does anyone have any experience (succesful or unsuccessful) with these
vectors?
Many thanks,
Silvia
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Silvia Onesti
Sincrotrone Trieste S.C.p.A.
SS14 - km 163,5 in AREA Science Park
34149 Basovizza, Trieste ITALY
Tel: +39 040 3758451
Mob: +39 366 6878001
Email: [email protected]
http://www.elettra.trieste.it/PEOPLE/index.php?n=SilviaOnesti.HomePage
http://www.sissa.it/sbp/web_2008/C_Structural_Biology.html
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