On 13:16 Wed 11 Nov , Matthias Zebisch wrote: > What is the optimal wavelength for Sulfur SAD phasing? > Is it 1.9A or should one go below that to reduce absorption/damage.
Some additional advice about factors other than wavelength: For S-SAD on a home Cu source, my previous lab showed that optimizing (NOT maximizing!) redundancy and using *lower* resolution were important. The reference: G. N. Sarma and P. A. Karplus. "In-house sulfur SAD phasing: a case study of the effects of data quality and resolution cutoffs." Acta Cryst. (2006). D62, 707-716. -- Thanks, Donnie Donald S. Berkholz, Postdoctoral research fellow James R. Thompson lab, Physiology & Biomedical Engineering Grazia Isaya lab, Pediatric & Adolescent Medicine Mayo Clinic College of Medicine 200 First Street SW Rochester, MN 55905
