Re: [ccp4bb] Enzyme inhibitor

[Ezra Peisach]

Sivaraman Padavattan wrote:
Dear all,
I am performing an assay to screen for the inhibitor molecules. One of 
our compound shows inhibition at 100 μM and activation at 500μM conc. 
What could be the possible reason for the compound work differently in 
two different conc.

Thanks
Sivaraman


     


Not saying this is right - but how about product inhibition 
(competition) and allosteric activation?

Ezra