You don't give details on the assay, but if it is a fluorescence or colorimetric screen, you might have interference. Is the compound precipitating and/or aggregating?
Good luck- Steve -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Ezra Peisach Sent: Monday, November 30, 2009 11:29 AM To: [email protected] Subject: Re: [ccp4bb] Enzyme inhibitor Sivaraman Padavattan wrote: > Dear all, > I am performing an assay to screen for the inhibitor molecules. One of > our compound shows inhibition at 100 μM and activation at 500μM conc. > What could be the possible reason for the compound work differently in > two different conc. > > Thanks > Sivaraman > > > > > Not saying this is right - but how about product inhibition (competition) and allosteric activation? Ezra Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
