Hi, This question may have been asked before, so please excuse me for re-posting it. I notice that when I do a round of 10 cycles of refmac combined with (5cycles, 3 sigma level, 1.4 A resolution) of findwaters in coot, some water molecules from the different coot cycles fall one on top of the other. Does that mean that coot does not check for clashes between water molecules detected in previous cycles ? And in any case, does coot put another water molecule in the same place (or nearly) because there is still residual difference density after placing the first one ? I assume that this happens here, which in fact can be beneficial in my current structure where I'm also trying to locate chloride ions (I don't have anomalous data in the correct wavelength so I can't depend on that).
Please advise. Thanks. Boaz Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel Phone: 972-8-647-2220 ; Fax: 646-1710 Skype: boaz.shaanan
