The obvious Q is whether there is some additive in the crystallisation
or cryo which could be showing up in the maps..
But in such a high symmetry space group is it possible that the blobs
are on some symmetry axis? I sometimes observe awful holes or peaks on 3
fold axes, where I think any "noise" feature isexaggerated by the symmetry.
Eleanor
Rajan wrote:
Dear All,
Happy New Year
I am working with a 2.06 Ang resolution structure in F432 space group and
further using REFMAC (also tried Phenix) program for refinement of my
model.
Every thing in the structure seems good, R factor/ R free= 18.59/20.77 but
the structure is too noisy (blobs of difference Fourier with red as well as
green density).
As such electron density is looking good for the protein part.
Is there any parameter which i had to check.
Thanking you in advance
Rajan
