Rajan, I'll second what Eleanor and George have said - high symmetry space groups with noisy Intensity measurements (perhaps it was a weak data set with high Rmerge?) really exaggerates the amount of "garbage" that you see in the maps. Cheers- Steve
________________________________ From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Rajan Sent: Wednesday, January 06, 2010 1:22 AM To: [email protected] Subject: [ccp4bb] Lots of noise in structure Dear All, Happy New Year I am working with a 2.06 Ang resolution structure in F432 space group and further using REFMAC (also tried Phenix) program for refinement of my model. Every thing in the structure seems good, R factor/ R free= 18.59/20.77 but the structure is too noisy (blobs of difference Fourier with red as well as green density). As such electron density is looking good for the protein part. Is there any parameter which i had to check. Thanking you in advance Rajan -- ************************************************ Current Address: Rajan Vyas Research Scholar Deptt. of Biotechnology Panjab University Chandigarh, India 160014 Mob. +919417374197 Fax: +91-172-2625254 ************************************************ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
