I have had very good results using 10% Tris-tricine gels, following the protocol in Current Protocols for our chemokines (~8,000 mw)
James W. Murphy, Ph.D. Associate Research Scientist; Dept. of Pharamcology Facility Manager; Macromolecular Crystallography Facility Yale University School of Medicine 333 Cedar Street, SHMB345 New Haven, CT 06510 Office: 203-737-1526 Cell: 203-906-5759 Fax: 203-737-2027 -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Jacob Keller Sent: Thursday, February 11, 2010 2:13 PM To: [email protected] Subject: [ccp4bb] SDS-PAGE of peptides Dear Crystallographers, does anybody have any good tricks for getting nice, sharp SDS-PAGE bands of peptides < 10kD? Regards, Jacob Keller ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: [email protected] *******************************************
