You can include urea in your gel to resolve ~5 kDa without using gradient gel. I am sorry that I forgot what was the percentage. Please take a look at "Gel Electrophoresis of Proteins: A Practical Approach". Or you can try the premade gradient gel from Invitrogen. http://www.invitrogen.com/etc/medialib/en/filelibrary/pdf.Par.99253.File.dat/O-063575-NuPage_fin.pdf. The Bis-Tris/Mes gel can resolve 2.5KDa peptide.
Regards, Nian Huang, Ph.D. Univ. of Texas Southwestern Medical Center On Thu, Feb 11, 2010 at 1:13 PM, Jacob Keller <j-kell...@md.northwestern.edu> wrote: > > Dear Crystallographers, > > does anybody have any good tricks for getting nice, sharp SDS-PAGE bands of > peptides < 10kD? > > Regards, > > Jacob Keller > > ******************************************* > Jacob Pearson Keller > Northwestern University > Medical Scientist Training Program > Dallos Laboratory > F. Searle 1-240 > 2240 Campus Drive > Evanston IL 60208 > lab: 847.491.2438 > cel: 773.608.9185 > email: j-kell...@northwestern.edu > *******************************************
