What is 1mM BTW? I am not familiar with this abbreviation.
PBS phoshate buffered saline (Phosphate + NaCl) not suitable for zinc
binding proteins
BBS borate buffered saline (Borate + NaCl) wrong pH for zinc binding.
BTW ? (? ? ?) No idea what this is. Charles, do you know if it includes
HEPES or Phosphate?
Enrico.
On Thu, 01 Apr 2010 11:06:10 +0200, Charles Allerston
<[email protected]> wrote:
Hi,
are you sure it is your protein precipitating? You will get a cloudy
precipitate appearing in HEPES and Phosphate buffers on addition of
ZnCl, without protein.
cheers
charlie
dengzq1987 <[email protected]> 3/31/2010 5:08 pm >>>
hello everyone, recently i purify a protein conteining zinc binding
domain,and i want to determine its structure.i get the crystal,but poor
diffraction.so i try to adding zinc into the protein to optimize the
crystal,but the protein precipitate immidiately even the znic is 1
mM.BTW,we use the protein to do zinc scan,we don't find the zinc. does
anyone have some advice?
2010-03-31
dengzq1987
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Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
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