I have no idea, it is not my buffer, it is dengzq1987's! 'BTW' means 'by the way', BTW.
cheers, charlie >>> Enrico Stura <[email protected]> 4/1/2010 11:01 am >>> What is 1mM BTW? I am not familiar with this abbreviation. PBS phoshate buffered saline (Phosphate + NaCl) not suitable for zinc binding proteins BBS borate buffered saline (Borate + NaCl) wrong pH for zinc binding. BTW ? (? ? ?) No idea what this is. Charles, do you know if it includes HEPES or Phosphate? Enrico. On Thu, 01 Apr 2010 11:06:10 +0200, Charles Allerston <[email protected]> wrote: > Hi, > > are you sure it is your protein precipitating? You will get a cloudy > precipitate appearing in HEPES and Phosphate buffers on addition of > ZnCl, without protein. > > > cheers > > charlie > >>>> dengzq1987 <[email protected]> 3/31/2010 5:08 pm >>> > hello everyone, recently i purify a protein conteining zinc binding > domain,and i want to determine its structure.i get the crystal,but poor > diffraction.so i try to adding zinc into the protein to optimize the > crystal,but the protein precipitate immidiately even the znic is 1 > mM.BTW,we use the protein to do zinc scan,we don't find the zinc. does > anyone have some advice? > > 2010-03-31 > > > > dengzq1987 -- Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab LTMB, SIMOPRO, IBiTec-S, CEA Saclay, 91191 Gif-sur-Yvette Cedex FRANCE http://www-dsv.cea.fr/en/ibitecs/82 http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html e-mail: [email protected] Fax: 33 (0)1 69 08 90 71
