Dear ALL:
Sorry for this kind of off-topic question.
I am going to co-crystallize one protein with cholesterol. I read some
papers saying that
their protein can be pre-incubated with 1mM cholesterol in the presence of 5%
(v/v) ethanol.
TO do so, I first dissolved cholesterol in 100% ethanol at a concentration
of 10mM. However, it is very difficult to make the dilution into 5% ethanol
either just in water or some buffers.
Does anyone have such experience to make cholesterol solution in normal
buffers plus some ethanol?
Thanks a lot,
Jerry McCully
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