Jerry,
Steroids have a certain solubility also in ethylene glycol and PEG.
You might be able to work out a crystallization strategy around this.
The ethanol/PEG combination was used in:
http://www.nature.com/nature/journal/v437/n7055/full/nature03923.html
Enrico.
Dear ALL:
Sorry for this kind of off-topic question.
I am going to co-crystallize one protein with cholesterol. I read
some papers saying that
their protein can be pre-incubated with 1mM cholesterol in the presence
of 5% (v/v) ethanol.
TO do so, I first dissolved cholesterol in 100% ethanol at a
concentration of 10mM. However, it is very difficult to make the
dilution into 5% ethanol either just in water or some buffers.
Does anyone have such experience to make cholesterol solution in
normal buffers plus some ethanol?
Thanks a lot,
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: [email protected] Fax: 33 (0)1 69 08 90 71
