Re: [ccp4bb] degradation of protein durring freez thaw

[Frank Niesen]

An aspect that is sometimes overlooked is that the need to avoid mini 
freeze-thaw cycles does not only call for a quick, snap-freeze process for 
samples (in thin-walled PCR tube and not too large volume; 50-100 ul I'd 
recommend), but - in turn - also for quick thawing: I prefer holding the PCR 
tube under running cold water straight after removal from the freezer, and 
stick into the ice bucket only after I see that all of the sample is thawed.
Best,
Frank