Sounds like good advice - I suppose that since pure water un-freezes last, you could have protein concentration inhomogeneities? So in areas where the protein concentration is raised it could start to feel like crashing out.
All the best Martyn Martyn Symmons Cambridge ----- Original Message ---- From: Frank Niesen <[email protected]> To: [email protected] Sent: Friday, 23 April, 2010 10:40:59 Subject: Re: [ccp4bb] degradation of protein durring freez thaw An aspect that is sometimes overlooked is that the need to avoid mini freeze-thaw cycles does not only call for a quick, snap-freeze process for samples (in thin-walled PCR tube and not too large volume; 50-100 ul I'd recommend), but - in turn - also for quick thawing: I prefer holding the PCR tube under running cold water straight after removal from the freezer, and stick into the ice bucket only after I see that all of the sample is thawed. Best, Frank
