Yes, this can be done, but you need buccaneer 1.4, which I haven't
released yet. We've seen significant benefits to preserving the heavy
atoms through to the refinement, although the code has been written so
that it can preserve DNA or any other know structure features as well.
I'll try and put together a release next week so you can try it out.
Kyle Dolan wrote:
Hello,
I am trying to use Buccaneer to improve the model of a protein/DNA
complex which I solved recently. However, my initial attempt failed
because the program deleted the DNA chains and rebuilt protein chains in
their density. Is there a way to specify that the expected outcome
should contain a DNA ligand--either in the input model pdb or the input
sequence file--and to instruct the program to only work on the protein
during building?
Thanks,
Kyle
Kyle T. Dolan
Department of Biochemistry and Molecular Biology
The University of Chicago
[email protected] <mailto:[email protected]>
