CCP4 way: locate the Se sites with SHELX (if you use the CCP4I gui it's technically a ccp4 program :-) ) Try using only your peak data set first. If you can't locate your sites with the single wavelength then add remote (DAD) and if that doesn't work go MAD.
non-ccp4 way run hkl2map as frontend to SHELX, do the same thing. After 10 minutes go and open a bottle of your favourite sparkling cider* and start tracing the structure. Jürgen * can be replaced by other sparkling product containing trace amounts of EtOH supplemented with flavours On May 19, 2010, at 12:01 PM, Qing Lu wrote: > Hi All, > > I am new to protein crystallography. I would like to know the steps involved > in solving a MAD dataset by using the program in CCP4 where you determine the > phases and then obtain the trace. The dataset is collected at 3 different > wavelengths (peak, inflection and remote) using Se-Met as the scatterer. The > crystals diffracted to resolution of 2 Angstrsoms and has a good anomalous > signal. > > Thanks, > > Qing Lu - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/
