Dear ALL:
I am sorry for this stupid question.
I guess bacterial expression system is still most popular in structural
biology.
If you get a very good soluble E.coli expression of a human protein without
disulfide bonds,
to what extent do you believe that the oligomerization state of this bacterial
expression will reflect the real physiological state of this protein in humans?
Can someone give comments or refer some literature?
Thanks a lot,
Jerry McCully
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