Hi -
did you express your secreted mammalian protein in the E.coli
periplasm? If so, and if the oligomerization state is well defined, eg
its a 4mer, 5mer or whatever, and not a mixed population, I would be
quite confident that this is the 'true' native state. But, I would
only be sure in the presence of a functional assay: is your bacterial-
made protein functional? Thats the main question you need to ask. I do
not think that literature can help you establish anything here, its a
case dependent issue.
Having said all these, a well behaved protein produced in E.coli
(stable soluble, monodisperse),
is most likely in a native state, and I would go ahead with
experiments, e.g. crystallization while checking if its functional ...
But, before attempting to publish, you need to prove its functional,
and ideally prove the relevance of oligomerization by mutagenesis
experiments of the interface as soon as you get the structure.
Finally, mammalian expression systems for secreted mammalian proteins,
are really good, easy to use, and relatively cheap due to the
widespread use of growth media from biologists, and your institute is
likely to have most of the equipment you need, in the cell biology
department.
Check out these (open access) papers:
A time- and cost-efficient system for high-level protein production in
mammalian cells.Aricescu AR, Lu W, Jones EY. Acta Crystallogr D Biol
Crystallogr. 2006 Oct;62(Pt 10):1243-50.
Eukaryotic expression: developments for structural proteomics.
Aricescu AR, et al and , Jones EY. Acta Crystallogr D Biol
Crystallogr. 2006 Oct;62(Pt 10):1114-24.A.
On May 28, 2010, at 23:02, Jerry McCully wrote:
A little update on my own project:
It is a secreted protein without any modification reported. Size
exclusion shows that it is an oligomer. CD spectrum shows well-
defined secondary structure.
It is stable and soluble.
It may have different states in diseases' condition.
Now I was trying to figure out the native state of this protein. As
told by folks on CCP4bb, Most likely, the bacterial expression
reveals its original state.
Am I right?
Thanks a lot,
Jerry
Date: Fri, 28 May 2010 11:40:31 -0700
From: [email protected]
Subject: [ccp4bb] to what extent bacterial expression reveals the
native oligomerization state of mammalian proteins.
To: [email protected]
Dear ALL:
I am sorry for this stupid question.
I guess bacterial expression system is still most popular in
structural biology.
If you get a very good soluble E.coli expression of a human
protein without disulfide bonds,
to what extent do you believe that the oligomerization state of this
bacterial expression will reflect the real physiological state of
this protein in humans?
Can someone give comments or refer some literature?
Thanks a lot,
Jerry McCully
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