Hi, well, actually this is availalbe as powder (HYClone), as it says
on the page.. (we have it made in our media kitchen on a regular
basis, but
not for huge scale... so i dont know about the prices..)
https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId=LA11074__10347&categoryId=82051&productId=11960716
HTH,
Tommi
On Jun 7, 2010, at 5:52 PM, Dima Klenchin wrote:
We have happily made a transition last year from using Invitrogen's
SFM medium and cellfectin to Insect-Xpress (Lonza) and
polyethyleneimine for transfection. We are moving several protein
targets to large-scale cultures and would consider cost-cutting
alternatives. For example, Invitrogen and Thermo Sci both offer media
in powder form at an attractive price. Has anyone made a systematic
comparison of these media? Any particular recommendations regarding
powder media?
Last I checked, no manufacturer offered serum-free powedered medium.
Fetal calf serum is very expensive - once you count in its cost, the
overall price of the media becomes comparable.
If your protein is secreted, serum-containing media are not even a
realistic choice. If it is intracellular, buying dry medium and
adding FBS work well: is a lot more hassle, is about 20% cheaper,
and usually gives slightly higher titers and expression levels. Of
all the common media we compared, TNM-FH works best for us with Sf9
cells. Switching to dry medium also means some initial investment:
bottles, filtration equipment and filters. So it all pays off only
of you need large scale cultures long-term.
If you are aware of a dry serum-free insect cells medium, please
let me know - I'd love to try it.
Dima
Tommi Kajander, Ph.D.
Structural Biology and Biophysics
Institute of Biotechnology
University of Helsinki
Viikinkaari 1
(P.O. Box 65)
00014 Helsinki
Finland
p. +358-9-19158903
tommi.kajan...@helsinki.fi
