Atul,

Your space group and cell are rather inconsistent, maybe if you fixed them 
it would help. If it is really trigonal, it could be merohedrally twinned, 
I think that REFMAC can now handle that automatically.

George

Prof. George M. Sheldrick FRS
Dept. Structural Chemistry,
University of Goettingen,
Tammannstr. 4,
D37077 Goettingen, Germany
Tel. +49-551-39-3021 or -3068
Fax. +49-551-39-22582


On Thu, 17 Jun 2010, atul kumar wrote:

> dear all
> I am trying to solve structure of data set at 1.9 A,r merge 9.3,it belongs
> to space group P3221 unit cell 160.78 157.32 135.62 90.0 90.0 90.0,i has no
> NCS ,i did tls as well but after water addition the r factor and r free is
> stuck at 22 and 25 respectively. kindly guide me how can i bring down the R
> factor.
> 
> Thankin you.
> 
> Regards
> Atul
> 

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