> > atul kumar wrote: > >> dear all >> I am trying to solve structure of data set at 1.9 A,r merge 9.3,it belongs >> to space group P3221 unit cell 160.78 157.32 135.62 90.0 90.0 90.0,i has no >> NCS ,i did tls as well but after water addition the r factor and r free is >> stuck at 22 and 25 respectively. kindly guide me how can i bring down the R >> factor. >> > Maybe they're "stuck" because the refinement is already as good as possible for these data? R-factors of 22/25 aren't unreasonable at 1.9A, so unless you see other problems with the structure (suspicious difference density blobs, missing residues, poor geometry, etc.), I'd stop worrying. Inspect the maps for missing pieces, feed the structure to the Molprobity server, fix as many problems as possible, do one last refinement run, and move on to the next experiment, or start writing a paper.
-Nat
