hi CCPeers The Matthews coefficient of my protein is 3 calculated with matthews-cell content analysis CCP4 programe with 87% confidence , but when doing the refinement the third molecular couldn't get into the unit cell because of too many clashes.Deletion of the clashed AA did not work well, Then I used two molecules in the unit cell, After refining with Refmac, I found that the R factor is 0.29, R free is 0.40.I believe the value can be better with the real space refinement.But the question is that can the calculated Matthews coefficient be wrong?
Best regards Xinghua Qin -- Xinghua Qin College of Biological Sciences No.2, Yuan Ming Yuan West Road Haidian District，Beijing，China，100094 Tel: +86-10-62732672
