Hi everyone:
Thanks for all the responses.
The Matthaws-cell content analysis programe in CCP4 package gives the
results: 47% solvent content and 3 molecules in asu with 87% confidence.
the space group is P3121. how to carry out self rotation function? can
phaser do that work? If there areTwo moleculars in asu ,no clashes and the
TF values are above 10.but if three, the clashes are too much and TF valur
is about five.
And how to calculate the solvent content? Is the calculated solvent content
with the Matthews-cell content analysis programe not always right?
Best regards
Xinghua Qin
On Fri, Jun 25, 2010 at 10:42 AM, Vineet Gaur <[email protected]>wrote:
> Hi
> It would b good if u can mention the solvent content and space group along
> with the no. of molecules in asu/
>
> u can carry out self rotation function to check the no of mol in asu
> best
> vineet
> On Fri, Jun 25, 2010 at 6:42 AM, xinghua qin <[email protected]> wrote:
>
>> hi CCPeers
>> The Matthews coefficient of my protein is 3 calculated with
>> matthews-cell content analysis CCP4 programe with 87% confidence , but when
>> doing the refinement the third molecular couldn't get into the unit cell
>> because of too many clashes.Deletion of the clashed AA did not work well,
>> Then I used two molecules in the unit cell, After refining with Refmac, I
>> found that the R factor is 0.29, R free is 0.40.I believe the value can be
>> better with the real space refinement.But the question is that can the
>> calculated Matthews coefficient be wrong?
>>
>> Best regards
>>
>> Xinghua Qin
>>
>> --
>> Xinghua Qin
>> College of Biological Sciences
>> No.2, Yuan Ming Yuan West Road
>> Haidian District，Beijing，China，100094
>> Tel: +86-10-62732672
>>
>
>
--
Xinghua Qin
College of Biological Sciences
No.2, Yuan Ming Yuan West Road
Haidian District，Beijing，China，100094
Tel: +86-10-62732672
