Hi Andreas, this is the way I do it:
I use the program SITUS to convert the volume (in some SITUS-friendly EM format) to a pdb bead model (vol2pdb). Then I'll input that PDB into CRYSOL to generate the theoretical scatter. I vaguely recall that Chimera has a SAXS function (not sure), but that might be worth googling as well. Cheers, Kushol Kushol Gupta, Ph.D. Research Associate Van Duyne Laboratory - HHMI/Univ. of Pennsylvania School of Medicine [email protected] 215-573-7260 / 267-259-0082 Dear all, the other day I obtained SAXS data from which a low-resolution structural model was calculated. The model is simpler/less complex than one of the same protein that we obtained with cryo-EM. Is there a way to estimate theoretical SAXS data from a cryo-EM reconstruction to compare with the obtained raw data? Is there a program that does for a reconstruction what CRYSOL does for pdbs? I understand that there would be a huge amount of handwaving involved, but it might help us reconcile our models. Thanks. Andreas -- Andreas Förster, Research Associate Paul Freemont & Xiaodong Zhang Labs Department of Biochemistry, Imperial College London http://www.msf.bio.ic.ac.uk
