Run your sequence through these predictors to get an idea of domain location and disorder.
Disorder predictor links http://dis.embl.de/ http://globplot.embl.de/ http://www.strubi.ox.ac.uk/RONN http://bip.weizmann.ac.il/fldbin/findex http://www.ist.temple.edu/disprot/predictorVSL2.php http://iupred.enzim.hu/ http://www.pondr.com/ http://bioinf.cs.ucl.ac.uk/disopred/disopred.html Darren On 29 July 2010 11:24, Albert Guskov <[email protected]> wrote: > Hi Vikrant, > I guess Xtalpred server might be of interest for you. > check it at http://ffas.burnham.org/XtalPred-cgi/xtal.pl > Cheers, > *Albert GUSKOV (Dr) *| Research Fellow | Division of Structural & > Computational Biology | Nanyang Technological University > Proteos 7-01, Biopolis Drive 61, Singapore 138673 Tel: (65) 6586-9690 > GMT+8h | Cell: (65) 8366-2779 | Email: [email protected] | Web: > www.ntu.edu.sg > > 2010/7/29 vikrant saa <[email protected]> > >> I want to do cloning of a 40 Kd protein in pRSETA, and pGEX-KT vector. >> I don't have any idea about protein solubility, its multimeric >> form, stability and disorder etc. There is nothing known in the literature >> also. Is there any software that can predict these parameters, so that i >> can decide which domain i need to clone for soluble and stable protein >> purification. >> *****Vikrant >> *** >> >> ------------------------------------------------------------------------------------------------------------------------ >> ***Junior Research Fellow (CSIR) * >> *Lab No. 101, Dr. Varma Lab* >> *Cancer Research Institute >> Advance Centre for Treatment, Research and Eduction in Cancer (ACTREC) >> Tata Memorial Hospital, Kharghar, Navi Mumbai-410210 * >> ##################################################################### >> >> >> >> > -- ********************************************************************** Dr. Darren Hart, Team Leader High Throughput Protein Lab Grenoble Outstation European Molecular Biology Laboratory (EMBL) ********************************************************************** www.embl.fr/research/unit/hart/index.html For funded access to ESPRIT construct screening via EU FP7 PCUBE: http://tinyurl.com/ydnrwg4 Email: [email protected] Tel: +33 4 76 20 77 68 Fax: +33 4 76 20 71 99 Skype: hartdarren Postal address: EMBL, 6 rue Jules Horowitz, BP181, 38042 Grenoble, Cedex 9, France **********************************************************************
