Tried with my protein and it says *very* difficult but I got crystals.
Quoting Albert Guskov <[email protected]>:
Hi Vikrant,
I guess Xtalpred server might be of interest for you.
check it at http://ffas.burnham.org/XtalPred-cgi/xtal.pl
Cheers,
*Albert GUSKOV (Dr) *| Research Fellow | Division of Structural &
Computational Biology | Nanyang Technological University
Proteos 7-01, Biopolis Drive 61, Singapore 138673 Tel: (65) 6586-9690 GMT+8h
| Cell: (65) 8366-2779 | Email: [email protected] | Web: www.ntu.edu.sg
2010/7/29 vikrant saa <[email protected]>
I want to do cloning of a 40 Kd protein in pRSETA, and pGEX-KT vector. I
don't have any idea about protein solubility, its multimeric form, stability
and disorder etc. There is nothing known in the literature also. Is there
any software that can predict these parameters, so that i can decide which
domain i need to clone for soluble and stable protein purification.
*****Vikrant
***
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***Junior Research Fellow (CSIR) *
*Lab No. 101, Dr. Varma Lab*
*Cancer Research Institute
Advance Centre for Treatment, Research and Eduction in Cancer (ACTREC)
Tata Memorial Hospital, Kharghar, Navi Mumbai-410210 *
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