Dear Jürgen, Thanks for the feedback.
Differential Scanning Fluorimetry is a nice method but my current goal is to identify the nature of the cofactor if any. I was wondering whether MassSpec could be of help. Sincerely, Xuan 2010/8/4 Jürgen Bosch <[email protected]> > Check the thermal stability with and without your ligand. You could do this > via CD or with the help of Sypro Orange in a RT-PCR machine. > Jürgen > > ...................... > Jürgen Bosch > Johns Hopkins Bloomberg School of Public Health > Department of Biochemistry & Molecular Biology > Johns Hopkins Malaria Research Institute > 615 North Wolfe Street, W8708 > Baltimore, MD 21205 > Phone: +1-410-614-4742 > Lab: +1-410-614-4894 > Fax: +1-410-955-3655 > http://web.mac.com/bosch_lab/ > > On Aug 4, 2010, at 4:10, Xuan Yang <[email protected]> wrote: > > > Dear All, > > > > 3D structure modeling server I-TASSER predicts a binding site for NADPH > and I want to test this prediction. What would be the nice quick way to tell > whether this protein bind NADPH or not, when I have a lot of recombinant > protein? > > > > Sincerely, > > > > Xuan Yang >
