Rui
Assuming that they are protein, this is a perfect candidate for "MMS" microseeding into random screens You can do it with a robot or by hand See D'Arcy et al. Acta Cryst. (2007). D63, 550 - 554. 'An automated microseed matrix-screening method for protein crystallization' A very good recent article is Obmolova et al. Acta Cryst. (2010). D66, 927-933 'Promoting crystallization of antibody-antigen complexes via microseed matrix screening' (See also our website at http://www.douglas.co.uk/mms.htm) Yibin, what proteases do you use, what conc, how much do you add? Patrick -- For information and discussion about protein crystallization and automation, please join our bulletin board at http://groups-beta.google.com/group/oryx_group?hl=en [email protected] Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of rui Sent: 25 August 2010 13:37 To: [email protected] Subject: [ccp4bb] turn granular to crystal Hi, All, I'm trying to crystallize a soluble protein and got something like granular, they are rounded shaped and not so regular and also don't have sharp edges. See the attached pic. The current condition is PEG4000 and pH around 5. How can I improve this condition? Thanks a lot.
