Dear Rui,
I can only support Patrick's view on MMS. We've got some very good
results with this technique in one tricky case in which the starting
point wasn't much better than yours.
In addition to the papers Patrick suggested the following might also
be worth a look:
The role of bias in crystallization conditions in automated
microseeding.
St John FJ, Feng B, Pozharski E.
Acta Crystallogr D Biol Crystallogr. 2008 Dec;64(Pt 12):1222-7.
Cheers
Roberto
On 25 Aug 2010, at 12:37, rui wrote:
Hi, All,
I'm trying to crystallize a soluble protein and got something like
granular, they are rounded shaped and not so regular and also don't
have sharp edges. See the attached pic. The current condition is
PEG4000 and pH around 5. How can I improve this condition? Thanks a
lot.
<granular.JPG>
---
Dr. Roberto Steiner
Randall Division of Cell and Molecular Biophysics
New Hunt's House
King's College London
Guy's Campus
London, SE1 1UL
Phone +44 (0)20-7848-8216
Fax +44 (0)20-7848-6435
e-mail [email protected]
