hi remember to reindex your data to P21212 in case you used Phaser to search all alternative orthorhombic SG's and it found P22121 Preben
On 03/10/2010, at 04.56, Jack Russel wrote: > Hi all, > > I have collected a data at 2.9 Å and the solved the structure using phaser . > the space group comes to be P2 21 21. There are 4 molecules in Assymetric > unit and an octamer is generated according to the symmetry. But after > repeated rounds of rigid body refinement with REFMAC5 and model building with > coot the R factor had been struck at 40% and R free at 50%. > > So my first question is whether my solution after phaser is correct. And if > it is how can i lower the R factor and Rfree. > > The second question is it possible to have such a large difference between R > factor and R free. > > Thanks in advance > >
