Is that necessary?
Eleanor
J. Preben Morth wrote:
hi
remember to reindex your data to P21212 in case you used Phaser to search all
alternative orthorhombic SG's and it found P22121
Preben
On 03/10/2010, at 04.56, Jack Russel wrote:
Hi all,
I have collected a data at 2.9 Å and the solved the structure using phaser . the space group comes to be P2 21 21. There are 4 molecules in Assymetric unit and an octamer is generated according to the symmetry. But after repeated rounds of rigid body refinement with REFMAC5 and model building with coot the R factor had been struck at 40% and R free at 50%.
So my first question is whether my solution after phaser is correct. And if it
is how can i lower the R factor and Rfree.
The second question is it possible to have such a large difference between R
factor and R free.
Thanks in advance
