Hello Dilip Badgujar, What exactly do you mean by "not working" when you lowered to concentration? Did you lower the complex concentration and at some point you got - no precipitation at all - no crystals but aggregate precipitation - something else?
You also do not mention that you tried lowering the precipitant concentration. In case you did, the same question as above applies. In case that you got to a point (by lowering the precipitant concentration) with no precipitation at all (clear drop) you could also try seeding. Cheers, Tim On Sun, Oct 10, 2010 at 06:15:44AM -0000, Dilip Badgujar wrote: > hi guys > > I am trying to crystallize protein complexed with peptide(14mer). > In initial screening i got microcrystals in following condition > - 0.2M MgCL2 > 0.1M Tris pH 8.5 > 28 % PEG 4000 > Ptotein concentration - 12.5mg/ml and peptide conc.-5mg/ml > Complex ratio- (Protein-Peptide)(1:1.5) > Incubation temp.-22 degree > Method - Sitting drop vapor diffusion method > > within four hours of setting trials I can see too many microcrystals. > Though i tried reduction of protein and precipitant conc. as well as using > mineral oil to decrease evaporation rate but still it is not working. > > I am waiting for your valuable suggestions. -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A
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