Hi,
What is the pI of your protein? If it happens to be anywhere near your buffer pH, then you should move away from it. Have you measured the size of you micro-crystals? You may be surprised what micro-diffraction can do for you these days. Good luck! Nukri Ruslan Sanishvili (Nukri), Ph.D. GM/CA-CAT Biosciences Division, ANL 9700 S. Cass Ave. Argonne, IL 60439 Tel: (630)252-0665 Fax: (630)252-0667 [email protected] ________________________________ From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Dilip Badgujar Sent: Sunday, October 10, 2010 1:16 AM To: [email protected] Subject: [ccp4bb] Too many microcrystals....... hi guys I am trying to crystallize protein complexed with peptide(14mer). In initial screening i got microcrystals in following condition - 0.2M MgCL2 0.1M Tris pH 8.5 28 % PEG 4000 Ptotein concentration - 12.5mg/ml and peptide conc.-5mg/ml Complex ratio- (Protein-Peptide)(1:1.5) Incubation temp.-22 degree Method - Sitting drop vapor diffusion method within four hours of setting trials I can see too many microcrystals. Though i tried reduction of protein and precipitant conc. as well as using mineral oil to decrease evaporation rate but still it is not working. I am waiting for your valuable suggestions. <http://sigads.rediff.com/RealMedia/ads/click_nx.ads/www.rediffmail.com/ signatureline....@middle?>
