Hi ivan, The detection senstivity of proteins depends on staining technique, You have not mentioned about staining, whether silver or Coomassie. The silver staining is more sensitive than coomassie, typically you can see 10-50 ng of a protein in silver staining. It does vary, however, with the glycosylation and physical properties of the protein.
Shivendra On 2 November 2010 08:20, xaravich ivan <xaravich.i...@gmail.com> wrote: > Hi everyone, > I have grown some crystals after micro-seeding starting from thin-small > needles from needle-clusters. These crystals are larger in size than the > needles but are comparable to the shape and don't look like salt crystals. > But I cannot see the bands( its a complex) in the SDS-PAGE.I do not have a > home source,handy and would like to send these to the synchrotron. > > Is it possible to NOT see a band of protein crystals in SDS-PAGE, if, say, > the amount of protein is < 1uG? > Has anyone experienced such a thing (no band in gel, but crystal > diffracts)? > It would be nice if I get observations/suggestions. > > ivan >
