Dear All and Peter, In one of my structure, I find similar positive density though my protein was not methylated. Are there are any other reasons for appearance of such positive density next to Lysine. Thanking you Yogi
From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of peter66 wright Sent: Thursday, November 04, 2010 7:26 AM To: [email protected] Subject: [ccp4bb] How to add methyl group on the NZ atom of lysine Dear All, Could anyone please tell me how to add methyl group in COOT on the NZ atom of lysine that has been methylated before crystallisation? The density map is attached to this mail from which you can clearly see the methyl group should be there, but I do not know how to add. Many thanks for help. Peter
