Hi Peter, Iff you are sure you are dealing with methyl-lysine, you can get it into your structure by using "Get monomer" to get the compound MLZ. Position it correctly and then merge it into the structure. I prefer using a text editor to do this. Make sure you put the ATOM records at the right position in the file (otherwise real-space refinement won't work). You probably also need to make LINK records, which you can copy from an existing PDB file (1xer has the links you need). Helix-Turn-Helix, Robbie Joosten
________________________________ > Date: Thu, 4 Nov 2010 09:04:53 -0400 > From: [email protected] > Subject: Re: [ccp4bb] How to add methyl group on the NZ atom of lysine > To: [email protected] > > > Dear All and Peter, > > In one of my structure, I find similar positive density though my > protein was not methylated. > > Are there are any other reasons for appearance of such positive density > next to Lysine. > > Thanking you > > Yogi > > > > From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of > peter66 wright > Sent: Thursday, November 04, 2010 7:26 AM > To: [email protected] > Subject: [ccp4bb] How to add methyl group on the NZ atom of lysine > > > > Dear All, > > Could anyone please tell me how to add methyl group in COOT on the NZ > atom of lysine that has been methylated before crystallisation? The > density map is attached to this mail from which you can clearly see the > methyl group should be there, but I do not know how to add. > Many thanks for help. > > Peter
