I've had good luck cryoprotecting high salt crystal conditions with sodium
malonate (2.0-2.5M). Start with a 5M sodium malonate solution and dilute to
40-50% with mother liquor.
good luck,
Rob
________________________________________
From: CCP4 bulletin board [[email protected]] On Behalf Of Jerry McCully
[[email protected]]
Sent: Wednesday, December 15, 2010 1:13 PM
To: [email protected]
Subject: [ccp4bb] cryoprotectant for protein crystal grown from Di-sodium
hydrogen phosphate
Dear All;
Recently we got some crystals from the condition #51 in the SaltRx
crystallization kit from Hampton research.
It contains 1.5M Na2HPO4 and 0.1M Tris(pH8.5). We am going to do a test
diffraction ASAP.
What cryoprotectant did you use for this condition?
Thanks a lot and have a nice holiday season!
Jinghua
