Quickly passing the crystal through Paratone N has worked well for me when I crystallize in ammonium sulfate or sodium citrate conditions. Another trick is to dissolve sucrose (table sugar) in 10uL of the reservoir solution until it is saturated. Then separate the sucrose-reservoir mix into two 5ul drops. Add 5ul of the reservoir solution to one of the drops to make a 50% solution. Pass the crystal through the 50% saturated sucrose solution, then the 100% saturated sucrose solution and freeze. Good luck with the crystal!
Bryan -------------------------------------------------------------------------- Confidentiality Notice: This message is private and may contain confidential and proprietary information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorized use or disclosure of the contents of this message is not permitted and may be unlawful. -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Robert Kirchdoerfer Sent: Wednesday, December 15, 2010 4:26 PM To: [email protected] Subject: Re: [ccp4bb] cryoprotectant for protein crystal grown from Di-sodium hydrogen phosphate I've had good luck cryoprotecting high salt crystal conditions with sodium malonate (2.0-2.5M). Start with a 5M sodium malonate solution and dilute to 40-50% with mother liquor. good luck, Rob ________________________________________ From: CCP4 bulletin board [[email protected]] On Behalf Of Jerry McCully [[email protected]] Sent: Wednesday, December 15, 2010 1:13 PM To: [email protected] Subject: [ccp4bb] cryoprotectant for protein crystal grown from Di-sodium hydrogen phosphate Dear All; Recently we got some crystals from the condition #51 in the SaltRx crystallization kit from Hampton research. It contains 1.5M Na2HPO4 and 0.1M Tris(pH8.5). We am going to do a test diffraction ASAP. What cryoprotectant did you use for this condition? Thanks a lot and have a nice holiday season! Jinghua
