If they look like crystals, why don't you shoot them and see if they diffract ? Some people have been lucky with this approach.
Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/<http://web.me.com/bosch_lab/> On Jan 19, 2011, at 5:36 PM, Jerry McCully wrote: Dear ALL; Recently I am purifying a cell surface receptor by refolding of its inclusion bodies. Actually I can purify some functional monomers of this protein. However, the protein precipitates during the concentration. Interestingly, the precipitates look like crystals but not heavy aggregates. I guess the problem is due to the missing of glycosylation. Does anyone have suggestions to increase the current solubility? Thanks a lot, Jerry McCully