OK, thanks for the answers so far. I think my question hasn't been precise enough. Of course I have already optimised the purification using the Mono S column. However, resolution is not perfect and in the near future I will have to generate a lot of material and would like to use a larger column. The Mono S beads come at > 1000 GBP per ml. S-HyperD is 110 GBP for 5 ml. I found some older papers that didn't find the Mono beads superior plus they have a binding capacity of about 85 mg/ml. That is why I am interested in using them and was wondering if anybody on this list has ever used HyperD (what a name) beads.
Best wishes, Jonas ---------------------------------------- Jonas Boehringer Department of Biochemistry University of Oxford South Parks Rd Oxford OX1 3QU United Kingdom On 26 Jan 2011, at 02:30, Artem Evdokimov wrote: > Mini-S is not quite what you're looking for. It's 3uM particles and has a > nice fat back pressure. Resource S-15 and Resource S-30 (with 15uM and 30uM > average particle size) may be what you need. > > You may also want to take a look at monolithic columns because they develop a > lot less back pressure than bead-based ones: > > http://www.dionex.com/en-us/webdocs/38386-Man-065122-06-ProSwift-Mono-AX-CX-Mar10.pdf > > Artem > > On Tue, Jan 25, 2011 at 2:06 PM, Jonas Boehringer > <[email protected]> wrote: > Dear All, > I am currently looking into various cation exchange resins for the separation > of mono-, di-, tri-, tetra-,... ubiquitin. So far I have been using a small > Mono S column but would ideally like to both scale up and increase > resolution. Before I started searching for this online I have never heard of > the above resins and was wondering whether anybody on this list has any > experience, maybe even comparative, with anything other than Mono beads > (which are really expensive as well). > > Many thanks in advance and best wishes, > Jonas > ---------------------------------------- > Jonas Boehringer > Department of Biochemistry > University of Oxford > South Parks Rd > Oxford OX1 3QU > United Kingdom > > > >
