James Swindell, UGeorgia had a presentation "Evaluating the role of data reduction approach on the success rate on Sulfur-SAS phasing for a moderately diffracting crystal" at PittCon, Oct 2009, comparing HKL, d*TREK, XDS, Proteum2 & Mosflm
MfG, rgds, Eric Phone: +49 (721) 50997-5311 / +31 (15) 2152-501 Mobile: +49 (173) 7000-615 ________________________________ Von: CCP4 bulletin board [mailto:[email protected]] Im Auftrag von Van Den Berg, Bert Gesendet: Freitag, 28. Januar 2011 14:38 An: [email protected] Betreff: Re: [ccp4bb] Merging data to increase multiplicity I have heard this before. I'm wondering though, does anybody know of a systematic study where different data processing programs are compared with real-life, non-lysozyme data? Bert On 1/28/11 7:58 AM, "Bosch, Juergen" <[email protected]> wrote: I was a bit reductive with my statement (iPhone....) The equation below is suppose to read: If you have bad data, then you need to process with XDS in order to get the maximum out of your data. Thanks Tim, Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ <http://web.me.com/bosch_lab/> On Jan 28, 2011, at 7:44 AM, Tim Gruene wrote: Dear Jürgen, is this an assignment operator or an equal sign? For if it's the latter it could read that the result of processing data with XDS are bad data, which is rather rude and probably not what you meant. Tim On Fri, Jan 28, 2011 at 06:55:43AM -0500, Jürgen Bosch wrote: Bad data = processing with XDS Jürgen ...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Jan 28, 2011, at 6:46, José Trincão <[email protected]> wrote: Hello all, I have been trying to squeeze the most out of a bad data set (P1, anisotropic, crystals not reproducible). I had very incomplete data due to high mosaicity and lots of overlaps. The completeness was about 80% overall to ~3A. Yesterday I noticed that I could process the data much better fixing the mosaicity to 0.5 in imosflm. I got about 95% complete up to 2.5A but with a multiplicity of 1.7. I tried to integrate the same data fixing the mosaicity at different values ranging from 0.2 to 0.6 and saw the trend in completeness, Rmerge and multiplicity. Now, is there any reason why I should not just merge all these together and feed them to scala in order to increase multiplicity? Am I missing something? Thanks for any comments! Jose José Trincão, PhD CQFB@FCT-UNL 2829-516 Caparica, Portugal "It's very hard to make predictions... especially about the future" - Niels Bohr ------------------------------------------------------------------------------------ Bruker AXS GmbH, Karlsruhe HRB 107524 Amtsgericht Mannheim, Umsatzsteuer-Ident.Nr. DE812037551, Geschäftsführer - Dr. Frank Burgäzy, Bernard Kolodziej, Stephan Franz Westermann ------------------------------------------------------------------------------------ Der Inhalt dieser E-Mail ist vertraulich und ausschliesslich fuer den bezeichneten Adressaten bestimmt. Wenn Sie nicht der vorgesehene Adressat dieser E-Mail oder dessen Vertreter sein sollten, so beachten Sie bitte, dass jede Form der Kenntnisnahme, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhalts dieser E-Mail unzulaessig ist. Wir bitten Sie, sich in diesem Fall mit dem Absender der E-Mail in Verbindung zu setzen. The information contained in this email is confidential. It is intended solely for the addressee. Access to this email by anyone else is unauthorized. If you are not the intended recipient, any form of disclosure, reproduction, distribution or any action taken or refrained from in reliance on it, is prohibited and may be unlawful. Please notify the sender immediately. ------------------------------------------------------------------------------------
