Dear all, I have a question regarding the refinement and density map. My protein is 261 amino acids long and crystalize very nicely with very high resolution . There is no multiple spot in the image and diffraction looks amazing. I collected a dataset at 1.38 resolution and the space group is P43, overall Rmerg is 0.02 (most likely the space group is P4122, but then the solvent content is less than 16% for one molecule in the assymmetric unit, that are unlikely), so I processed the image in P4 (36% solvent) and run molecular replacement with a model that have 42 sequence identity. I got a solution in P43 that are good enough in some part but in the map there are many gap at even lower sigma level. I tried to refine and Rfree stacked at 36 along with Rwork, which is 33.
I also checked the degradation patteren of the protein in the crystal and it looks not degraded and full length protein crystalize. Is there anybody can suggest me anything that I can try? Regards, Md. Munan ShaikPhD Student Department of Biotehnology School of Bioscience and Biotechnology via G. Colombo 03 Padova 35131, Italy Mobile: 00393275671896 E-mail: [email protected] [email protected]
