Well, I've actually had success using phaser for placing 6 monomers in the a.u. P41212 space group BUT only after the first monomer was fully built into the initial map (pdb entry 2pan), in other words when the probe was 100% identical in sequence and structurally close enough to the target. The PTS peak height in the Patterson was ~60% of the origin at 0,0,~0.35). Earlier MR searches in phaser, using models that were 25-28% identical, picked up only 3-4 monomers.
Cheers, Boaz ----- Original Message ----- From: Phil Jeffrey <[email protected]> Date: Thursday, February 10, 2011 0:28 Subject: Re: [ccp4bb] Let's talk pseudotranslational symmetry (or maybe it's bad data). To: [email protected] > Is there a program that does ? I was under the impression > that they > were all equally good/bad at this, because any solution that > agrees with > the PTS has quite a high score and any solution that doesn't has > a low > score, irrespective of the correctness of the placement of the > molecules. > In one case that ritually defeats me with quite strong pseudo- > centering, > this seems to be true for heavy atom searches also. > > Phil Jeffrey > Princeton > > On 2/9/11 5:08 PM, Jon Schuermann wrote: > > I would NOT use Phaser for MR with PTS present. It doesn't > handle it > > correctly yet, since the likelihood targets don't account for PTS. > > Others may be able to explain it better. > Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel Phone: 972-8-647-2220 ; Fax: 646-1710 Skype: boaz.shaanan
