Dear all,
We are working on a Category 3 protein which must be contained so we have
our crystals mounted in a loop and then covered with a plastic Mitegen
cover which is glued in place. We're currently collecting at room
temperature, but wondered if anyone has any experience of using a contained
system at low temperatures? Any attempts I've had so far at freezing
through either the plastic or a glass capillary have resulted in formation
of ice on the surface so it is not even possible to see the crystal to
centre it.
Best wishes,
Becky
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Dr Becky Conners
School of Biochemistry
University of Bristol, UK
http://www.bris.ac.uk/biochemistry/brady
[email protected]
0117 3312149
