Mitegen plastic capillaries can actually last about a week from a Mitegen seminar I attended. I also got ice when I tried to freeze it once and I didn't further pursue it. Maybe coat the capillary with glycerol will help?
Nian Huang, Ph.D. UT Southwestern Medical Center On Thu, Feb 17, 2011 at 11:03 AM, R Conners, Biochemistry <[email protected]> wrote: > Dear all, > > We are working on a Category 3 protein which must be contained so we have > our crystals mounted in a loop and then covered with a plastic Mitegen cover > which is glued in place. We're currently collecting at room temperature, but > wondered if anyone has any experience of using a contained system at low > temperatures? Any attempts I've had so far at freezing through either the > plastic or a glass capillary have resulted in formation of ice on the > surface so it is not even possible to see the crystal to centre it. > > Best wishes, > > Becky > > ------------------------------------------------- > Dr Becky Conners > School of Biochemistry > University of Bristol, UK > > http://www.bris.ac.uk/biochemistry/brady > [email protected] > 0117 3312149 >
