Possibly deamidation of the protein, in particluar one or more lysines. What does the Mass spec look like? Cheers, Steve
________________________________ From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Ulli Hain Sent: Friday, February 18, 2011 12:14 PM To: [email protected] Subject: [ccp4bb] off-topic: 2 peaks on Cation Hi, I was wondering if anyone had possible explanations for a recombinantly expressed soluble protein that runs as 2 equal, slightly overlapping peaks on a cation exhanger but as one peak on a size exclusion column and same electrophoretic mobility on SDS-PAGE. -Ulli Adelaide Ulricke Hain PhD Candidate Johns Hopkins Bloomberg School of Public Health Department of Biochemistry and Molecular Biology 615 North Wolfe Street Baltimore, MD 21205 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
