Typo! I actually meant deamidation.
Nadir
Pr. Nadir T. Mrabet
Structural& Molecular Biochemistry
Nutrigenex - INSERM U-954
Nancy University, School of Medicine
9, Avenue de la Foret de Haye, BP 184
54505 Vandoeuvre-les-Nancy Cedex
France
Phone: +33 (0)3.83.68.32.73
Fax: +33 (0)3.83.68.32.79
E-mail: Nadir.Mrabet<at> medecine.uhp-nancy.fr
On 18/02/2011 20:36, Christian Roth wrote:
Hi,
you did not mention anything about your protein, but if it shows a metal
dependency, than different amounts of the metal ion might changen the overall
charge and influence the interactions with the ion exchanger. There might be
also a modification of an aminoacid f.e. decarboxylation of an aspartate or
glutamate.
Christian
Am Freitag 18 Februar 2011 18:13:36 schrieb Ulli Hain:
Hi, I was wondering if anyone had possible explanations for a
recombinantly expressed soluble protein that runs as 2 equal, slightly
overlapping peaks on a cation exhanger but as one peak on a size exclusion
column and same electrophoretic mobility on SDS-PAGE. -Ulli
Adelaide Ulricke Hain
PhD Candidate
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry and Molecular Biology
615 North Wolfe Street
Baltimore, MD 21205
