At 05:39 PM 2/25/2011, Pete Meyer wrote:
Or could anyone suggest a program that would be of help?
CAD scaling with a scale factor of 1.0 and negative B-factor (isotropic or
anisotropic) should do the trick. I haven't had much luck with density
sharpening (at least at ~4-5 Angstroms), but others have apparently had
some success with it.
Alternatively, CCP4i task Run FFT does the job:
1. Take MTZ from Refmac output
2. Run FFT to create simple map with SigmaA-weighted phases (i.e., PHWT
label).
3. In Infrequently used options, "Apply B-factor scaling to F1", specify
negative B-factor scaling value, usually within -10 to -50.
- Dima
