> 2) Scale F-holo and F-apo. Use "Experimental Phasing", "Data > Preparation", "Scale and Analyse Data Sets", "Scale refinement using > Scaleit". Don't include anomalous differences unless your interest is in > changing anomalous scatterers. My notes indicate that Fhscal works > better but does not have anisotropic scaling. If your two data sets do > not differ anisotropically try Fhscal.
This is not a problem. First scale anisotropically with your favourite program. Then the Kraut scaling correction using FHSCAL is a small correction on top of this, so just rescale the anisotropically-scaled output using FHSCAL. I didn't include an anisotropic scaling option in FHSCAL for the simple reason that this option was already available in other programs. > The greater the difference in cell constants the greater the "noise" in the > map. I think the high resolution cutoff for the maps should be 2 A > delta/(A+delta) where A is the cell edge with the largest change, and delta > is the amount of change (in Angstrom). > Basically a 1A change for a 100A edge would require a 2A resolution limit. > A 5A change would imply a 10A cutoff and a very boring map. I would > appreciate feedback on this procedure, if you find it hard to understand or > it doesn't work. Certainly the Phenix solution looks simpler. Dale See this thread: http://www.mail-archive.com/[email protected]/msg15533.html -- Ian
